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In-vivo Services

 


In vivo T-cell activation assay


Our DO11 in-vivo T-cell activation model is a robust mouse model that is relatively quick (3 days) and amenable for evaluation of compounds in-vivo to assess drug effect on T-cell and antigen presenting cell (e.g., dendritic cells) functions. DO11 transgenic mice express the ovalbumin-specific T-cell receptor. Treated mice receive a bolus of ovalbumin protein/peptide injection and plasma cytokine levels are monitored over time. The model is ideal for determining compound MOA or confirming proposed MOA targeting T-cells and/or antigen presenting cells. This model is also useful in in-vitro settings to evaluate compound effects for higher throughput.

INFLAMMATION MODELS


Rheumatoid arthritis mouse model


Our mouse collagen-induced arthritis (cia) is a robust and reliable model for efficacy testing of compounds. Male or female DBA/1 mice are immunized with type II collagen emulsified in Complete Freund's Adjuvant (CFA) (Day 1-20) and with type II collagen emulsified in Incomplete Freund's Adjuvant (IFA) three weeks later (Day 21). Prophylactic, semi-therapeutic or therapeutic treatment may be administered by oral gavage, intravenous injection, subcutaneous, intraperitoneal injection and/or implant with an osmotic pump. Controls available inlcude: anti-TNF biologics and/or dexamethasone. Clinical scores include a) Paw scores, b) Hind-paw thickness and c) Body weight. Independent histopathological analysis of joints is available at request and provided by a certified pathologist.

IN VIVO EFFICACY


Drug efficacy testing in rodents


Below are samples of in vivo models we provide at InTouch Bio. Our 24/7 secured facility is attended by a skilled staff with many years of experience in proper handling of research animals. Please contact us to speak with a scientist to discuss your particular study design and needs and we will quickly provide you a quote and associated timeline.



Animal care and proper use


We are committed to the proper care and use of animals in research. In addition to having an IACUC (institutional animal care and use committee), we are approved by the California Department of Public Health to work exclusively with laboratory rats and mice.

CANCER MODELS


Mouse tumor models


1) Syngeneic* subcutaneous tumor models

2) Syngeneic* lung metastasis cancer models (e.g., Lewis lung carcinoma LLC)

3) Xenograft subcutaneous tumor models in immunodeficient mice (e.g., SCID, Nude)


*Syngeneic tumor models employ animals with an intact immune system and thus ideal for evaluating test articles that modulate the immune response.

OBESITY MODEL


Mouse obesity animal model


Diet-induced obese (DIO) mouse model. Summary: C57BL/6 obese mice are allowed to acclimate over a period of 2 weeks while on a high-fat diet and enrolled into treatment groups when body weights stabilize and/or exhibit an upward trend. Mice are kept on a high-fat diet during treatment and weight recorded. Treatment route will vary as per client request. Terminal procedures performed (e.g., blood (plasma) collection, tissue harvest) will vary as per client request.